Solubilities of urea-crystallized tuna oil methyl esters in supercritical carbon dioxide were determined by a flow through extraction reactor. Experimental results obtained under a quasi-equlibrium condition showed that at 150 bar, solubilities of the esters in supercritical CO©ü were 0.075, 0.028 and 0.006 (w/w) at 40 , 60¡É and 80¡É , respectively. In the pressure and temperature ranges (100¡200 bar and 40¡80¡É ), the solubility increased with the density of CO©ü. However, selectivity of supercritical carbon dioxide on the extracted compounds was much better at low density than at high density. Supercritical fractionation with a temperature gradient (50¡75¡É ) resulted in concentrates of EPA and DHA in purifies of 12% and 85%, respectively.
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